Journal:

Article Title: Hematopoietic progenitors express neural genes

doi: 10.1073/pnas.2434383100

Figure Lengend Snippet: Immunocytochemical detection of neural gene expression in a subset of adult mouse whole ex vivo bone marrow (see Materials and Methods). Double immunocytochemical detection of neurofilament H and Pax-6 was performed in the same subset of bone marrow cells, and expression of neuronal neurofilament H, NeuN, and HuC/HuD was present in a subset of CD34+ bone marrow cells. GAD65, an enzyme responsible for synthesis of a major neurotransmitter, also was present in a subset of bone marrow cells. Oligodendroglial CNPase was present in a subset of bone marrow cells, whereas the astroglial marker GFAP was not detected on ex vivo bone marrow. Neurofilament H and Oct-4 were found in the same subset of ex vivo bone marrow cells. DAPI stains of the nuclei of all cells are shown.

Article Snippet: Cells were then treated with 0.25% Tween 20 for 3 min at 21°C, washed three times in PBS, and analyzed by standard immunocytochemistry methodology with the following antibodies: primary antibodies CD34 (553731, Pharmingen), Sca-1 (557403, Pharmingen), AA4.1 (559158, Pharmingen), cKit (CBL1359, Cymbus Biotechnology, Chandlers Ford, England), H-2K (553567, Pharmingen), CD45 (553076, Pharmingen), F4/80 (MCAP497, Serotec), Pax-6 (sc-11357, Santa Cruz Biotechnology), Oct-4 (sc-9081, Santa Cruz Biotechnology), Hu-antigen C/Hu-antigen D (HuC/HuD; A-21275, Molecular Probes), neurofilament H (SMI 312, Sternberger Monoclonals, Lutherville, MD; AB1989, Chemicon), neuronal nuclear protein (NeuN; MAB377, Chemicon), glutamic acid decarboxylase 65 (GAD65; AB5082, Chemicon), M2 muscarinic acetylcholine receptor (AB166–50UL, Chemicon), glial fibrillary acidic protein (GFAP; MAB3402, AB5040, and AB5804, Chemicon), 2′,3′ cyclic nucleotide 3′-phosphohydrolase (CNPase; MAB326, Chemicon), myelin oligodendrocyte-specific protein (MOSP; MAB328, Chemicon), NG2 chondroitin sulfate proteoglycan (A B5320, Chemicon), galactocerebroside (AB142, Chemicon), oligodendrocyte-marker antibody O4 (MAB345, Chemicon), MAG (myelin-associated glycoprotein; MAB1567, Chemicon), and proteolipid protein (PLP; MAB388, Chemicon).

Techniques: Expressing, Ex Vivo, Marker

Journal: Oncology reports

Article Title: Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model.

doi: 10.3892/or.2013.2772

Figure Lengend Snippet: Figure 5. Determination of stemness-associated activity and stem cell markers. (A) Representative images from the sphere formation assays. (B) Quantification of the sphere numbers. Values are the means ± SD of triplicate samples from 3 independent assays. *p<0.05; **p<0.01; ***p<0.001. (C) FACS analysis of the stem cell marker CD133. (D) Immunoblot analysis of the stem cell markers Oct4, Nanog and Bmi1.

Article Snippet: The antibodies used in this study included Snail (3895; Cell Signaling Technology, Inc.), Slug (ab27568; Abcam), Twist (sc-15393; Santa Cruz Biotechnology, Inc.), vimentin (550513) and fibronectin (610078; both from BD Biosciences), GAPDH (MAB374; Millipore), FAK (sc557), Tyr 925-phosphorylated FAK (sc-11766) and Oct4 (sc-9081) (all from Santa Cruz Biotechnology, Inc.), Nanog (ab21624; Abcam) and Bmi1 (2830; Cell Signaling Technology, Inc.).

Techniques: Activity Assay, Marker, Western Blot